ABSTRACT
To investigate the active compounds from on the heart and brain of mice at simulated high altitude.Fifty healthy male adult BALB/c mice were randomly divided into normal control group, hypoxic model group, acetazolamide group, petroleum ether extract of (PESI) group and octacosan group with 10 mice in each group. Acetazolamide group, PESI group and octacosan group were treated with acetazolamide PESI (200 mg/kg) or octacosan by single tail vein injection, respectively. Except normal control group, the mice were exposed to a simulated high altitude of for in an animal decompression chamber. After the mice were sacrificed by cervical dislocation, the heart and brain were histologically observed by HE staining; superoxide dismutase (SOD) activity, total anti-oxidant capacity (T-AOC) and the content of malondialdehyde (MDA) in plasma, heart and brain tissues were detected by WST-1 method, ABTS method and TBA method, respectively; lactic acid and lactate dehydrogenase (LDH) activity in plasma, heart and brain tissues were detected by colorimetric method and microwell plate method, respectively; ATP content and ATPase activity in heart and brain tissues were detected by colorimetric method. PESI and octacosane significantly attenuated the pathological damages of heart and brain tissue at simulated high altitude; increased SOD activity, T-AOC and LDH activity, and decreased the contents of MDA and lactic acid in plasma, heart and brain tissues; increased the content of ATP in heart and brain tissues; increased the activities of Na-K ATPase, Mg ATPase, Ca ATPase and Ca-Mg ATPase in myocardial tissue; and increased the activities of Mg ATPase, Ca-Mg ATPase in brain tissue. PESI and octacosan exert anti-hypoxic activity by improving the antioxidant capacity, reducing the free radical levels, promoting the anaerobic fermentation, and alleviating the energy deficiency and metabolic disorders caused by hypoxia in mice.
Subject(s)
Animals , Male , Mice , Altitude , Brain/metabolism , Heart , Malondialdehyde , Mice, Inbred BALB C , Superoxide Dismutase/metabolismABSTRACT
Aim To investigate the effect of petroleum ether extract of Eclipta prostrata(PEEEP) on biochem-ical parameters such as blood glucose, insulin, total glycerides,total cholesterol,creatinine,urea nitrogen, uric acid levels and histopathology of kidney in STZ in-duced diabetic rats. Methods The model of type 2 diabetic rats was established by feeding with high ener-gy diet for 8 weeks and injecting streptozotocin(30 mg ·kg-1), after making the model successfully, the rats were randomly divided into model control group, metformin positive control group and experimental group, and gavaged with distilled water, metformin (400 mg·kg-1) and different dose of PEEEP (100, 200,400 mg·kg-1) for 4 weeks, respectively. The contents of blood glucose,insulin,total glycerides,to-tal cholesterol, creatinine, urea nitrogen, uric acid were detected by corresponding kit, the pathological changes of kidney were observed by light microscopy, and the expression of TNF-α protein was detected by Western blot. Results Compared with model control group, PEEEP could reduce blood glucose, total glyc-erides, total cholesterol, creatinine, urea nitrogen, u-ric acid and increase the content of insulin in a dose-dependent manner (P <0.01 or P <0.05). It also could alleviate the pathological damage of kidney tis-sues and down-regulate the expression of TNF-α in kidney tissues. Conclusions PEEEP could effectively improve related biochemical parameters in streptozoto-cin induced diabetic rats, and it has protective effect on the kidney, providing the theoretical basis for fur-ther exploitation and utilization of Eclipta prostrata.